Skeletal Muscle Tissue Engineering Which Cell to Use

SCs are a common cell type utilized for tissue. Damage to skeletal muscle and subsequent loss of function due to aging trauma and disease has spurred the development of new tissue engineering and regenerative medicine approaches.

Muscle Tissue Of Human Body Skeletal Muscle Anatomy Skeletal Muscle Muscle Anatomy

PDF Introduction Tissue engineering is an innovative field with enormous developments in recent years.

. In our lab these generally consist of murine myoblast progenitor cells isolated from murine hind limb muscles or a murine myoblast cell line C2C12 mixed with a mixture of collagenMatrigel and plated between two anchoring points mimicking the muscle ligaments. In the early 1960s Mauro. Engineering models of human skeletal muscle tissue provides unique translational opportunities to investigate and develop therapeutic strategies for acute muscle injuries and to establish personalised and precision medicine platforms for in vitro studies of severe neuromuscular and musculoskeletal disorders.

Coculture with Skeletal Muscle Cells. Engineered muscle tissues can be used for several different purposes which include the production of tissues for use as a disease model in vitro eg. Skeletal muscle tissue is characterized by high metabolic requirements defined structure and high regenerative potential.

A new alternative approach to addressing difficult tissue reconstruction is to engineer new tissues. Skeletal muscle tissue engineering SMTE is a promising alternative to traditional VML surgical treatments that use autogenic tissue grafts and rather uses isolated stem cells with myogenic potential to generate de novo skeletal muscle tissues to treat VML. Satellite cells can be isolated from skeletal muscle tissue and subsequently used for studies into skeletal muscle cellular behavior biology or indeed engineering in vitro skeletal muscle.

This latter development has been a long forthcoming expectation for these cells and represent an exciting development in the field of tissue engineering. Many attempts to use tissue engineering for skeletal muscle wound repair have focused on incorporation of isolated musclederived cells such as myoblasts or. As such it constitutes an appealing platform for tissue engineering to address volumetric defects as proven by recent works in this field.

2009 developed a collagen sponge with different combinations of collagens for skeletal muscle tissue engineering. Skeletal muscle with in vivo functionality requires the cooperation of major types of tissues including muscle tissue nervous tissue vascular tissue and connective tissue. Tissue-engineered skeletal muscle is urgently required to treat a wide array of devastating congenital and acquired conditions.

Selection of the appropriate cell type requires consideration of several factors which amongst others include accessibility of the cell source in vitro myogenicity at high efficiency with the ability to maintain differentiation over extended. Although those tissue engineering techniques attempting regeneration of human tissues and organs have recently entered into clinical practice the engineering of skeletal muscle tissue ist still a scientific challenge. Potential stem-progenitor cell sources include and are not limited to satellite cells myoblasts mesoangioblasts pericytes muscle side-population cells CD133 cells in addition to embryonic stem cells mesenchymal stem cells amniotic fluid stem cells and induced pluripotent stem iPS cells.

Also based on the possibility of processing parallel. In vivo muscle regeneration is a multistep process that begins with the activation of a population of quiescent muscle-resident stem cells called. When these cells are isolated from skeletal muscle fibers they are termed muscle progenitor or precursor cells MPCs and can be cultured in a similar manner to any other cell.

Loss of skeletal muscle profoundly affects the health and well-being of patients and there currently is no way to replace lost muscle. Due to its tissue-specific regenerative potential the satellite cell has been identified as the ideal progenitor cell for skeletal muscle tissue engineering 3 4. We believe that a key step in the development of a prosthesis for reconstruction of dysfunctional muscular tissue is the ability to reconstitute the in vivo-like 3-dimensional 3D organization of skeletal muscle in vitro with isolated satellite cells.

13 Significant focus has been directed toward engineering the extracellular matrix ECM which plays a crucial role not only in the maintenance of muscle structure in vivo but. A Muscle cells and other supporting cells are combined with biomaterials in vitro followed by transplantation either after extended culture to promote muscle formation or immediately. Collagen is a promising material for skeletal muscle tissue engineering because it mimics the natural extracellular matrix thereby contributing to myoblast proliferation and differentiation.

IPSCs can be generated from somatic cells utilizing Yamanaka factors Oct34 Sox2 Klf4 c-Myc and allowed to undergo differentiation for individualized patient-specific treatment of diseases Kodaka et al 2017. Department of Chemistry Materials and Chemical Engineering Giulio Natta Politecnico di Milano Milano Italy. Alternative cell lines such as L6 rat myoblasts 4 neonatal muscle.

B Biomaterials either alone or combined with cytokines growth factors or cells secreting paracrine signals are delivered to. Skeletal muscle tissue engineering SMTE is a promising alternative to traditional VML surgical treatments that use autogenic tissue grafts and rather uses isolated stem cells with myogenic potential to generate de novo skeletal muscle tissues to treat VML. Potential stem-progenitor cell sources include and are not limited to satellite cells myoblasts mesoangioblasts pericytes muscle side-population cells CD133 cells in addition to embryonic stem cells mesenchymal stem cells amniotic fluid stem cells and induced pluripotent stem iPS cells.

Several myogenic and non-myogenic cell types. To study pressure ulcers for regenerative medicine and as a meat alternative 1The first reported 3D muscle constructs have been made many years ago and pioneers in the field are Vandenburgh and colleagues 23. Satellite cells are the native precursors to skeletal muscle tissue and are thus the most commonly studied starting source.

23 Cell sources for skeletal muscle tissue engineering In addition to the scaffold composition and design the source of muscle progenitors is a critical choice in tissue engineering approaches. Two general approaches of skeletal muscle tissue engineering. However they are challenging to expand in vitro generating an insufficient number of cells for tissue-engineering purposes.

Other cells may be considered as well eg. Thus a skeletal muscle is infused by capillaries and connected to nerve branches whereas several connective tissues cover the whole muscle. Satellite cells are the native precursors to skeletal muscle tissue and are thus the.

These advances are not only in the. For skeletal muscle tissue engineering satellite cells were initially considered to be the best candidate for cell therapy. The relative merits and inherent limitations of these cell types within the field.

Find read and. A range of cell types have been utilized for skeletal muscle tissue engineering including SCs myoblasts muscle-derived precursor cells MDPCs mesenchymal stem cells MSCs perivascular stem cells PSCs adipose-derived stem cells ASCs and induced pluripotent stem cells iPSCs 205206207.

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